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author:

党占国 (党占国.) [1] | 夏平安 (夏平安.) [2] | 高进勇 (高进勇.) [3] | 尹彦涛 (尹彦涛.) [4] | 王建举 (王建举.) [5] | 崔保安 (崔保安.) [6] | 陈溥言 (陈溥言.) [7]

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CQVIP PKU CSCD

Abstract:

根据GenBank公布的猪繁殖与呼吸综合征病毒(PRRSV)ATCC VR-2332株GP5基因序列设计1对特异性引物,应用RT—PCR方法扩增PRRSVHn/06-1分离株GP5基因片段.将扩增片段克隆到真核表达载体pcDNA3.0中,测序后用DNAstar序列分析,构建的pcDNA—GP5重组质粒瞬时转染293T细胞,48h后用Western-blot检测。证明GP5基因在293T细胞中获得了表达,可与PRRSV阳性血清发生特异性反应.

Keyword:

GP5基因 克隆 猪繁殖与呼吸综合征病毒 真核表达

Community:

  • [ 1 ] 河南农业大学牧医工程学院,河南郑州450002
  • [ 2 ] 福州大学,福建福州350002
  • [ 3 ] 南京农业大学,江苏南京210095

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Source :

河南农业大学学报

ISSN: 1000-2340

Year: 2008

Issue: 2

Volume: 42

Page: 196-199

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 0

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