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author:

管玉霞 (管玉霞.) [1] | 刘志琼 (刘志琼.) [2]

Indexed by:

CQVIP CSCD

Abstract:

根据小单孢菌产生庆大霉素的生物合成机理,利用基因克隆方法从棘孢小单孢菌(Micromonospora echinospora)基因组中扩增出庆大霉素生物合成的关键酶基因—2-脱氧青蟹肌糖合成酶基因(GntB),并将其通过大肠杆菌/链霉菌穿梭质粒pIJ699转化原菌株,采用硫链丝菌素抗性基因启动子带动2-脱氧青蟹肌糖合成酶基因在棘孢小单孢菌细胞中实现了转化。

Keyword:

2-脱氧青蟹肌糖合成酶基因(GntB) pIJ699 克隆 棘孢小单孢菌 转化

Community:

  • [ 1 ] 福州大学生物科学与工程学院,福州350002

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Source :

世界科技研究与发展

ISSN: 1006-6055

Year: 2009

Issue: 3

Volume: 31

Page: 417-419

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 1

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