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author:

李奕雅 (李奕雅.) [1] | 陈凡 (陈凡.) [2] | 汪少芸 (汪少芸.) [3] | 叶秀云 (叶秀云.) [4] | 陈躬瑞 (陈躬瑞.) [5] | 饶平凡 (饶平凡.) [6]

Abstract:

本课题利用亲和色谱和凝胶过滤色谱法从花生种子中分离得到了几丁质酶.经SDS-PAGE鉴定,该酶蛋白已达电泳纯,分子量约34.4 kD.在还原和非还原状态下均显示单一区带,表明其为单体蛋白.此外,通过等电聚焦法可测得该酶等电点为5.1.而酶学性质的相关研究则表明:该酶最适pH为5.4,最适反应温度为40~50℃.

Keyword:

几丁质酶 纯化 花生 表征

Community:

  • [ 1 ] 漳州师范学院生物科学与技术系
  • [ 2 ] 福州大学生物科学与工程学院

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Source :

漳州师范学院学报(自然科学版)

Year: 2008

Issue: 03

Page: 79-83

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ESI Highly Cited Papers on the List: 0 Unfold All

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Chinese Cited Count:

30 Days PV: 1

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