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author:

杨捷 (杨捷.) [1] | Allan M Showalter (Allan M Showalter.) [2]

Indexed by:

PKU CSCD

Abstract:

为确定拟南芥中一个经典阿拉伯半乳糖蛋白(arabinogalactan-protein,AGP)的亚细胞定位,克隆了这个基因的编码区,与绿色荧光蛋白(GFP)基因构建融合表达载体,经农杆菌介导转化烟草BY-2悬浮细胞.利用激光扫描共聚焦显微镜,在稳定转化的BY-2细胞表面观察到绿色荧光.研究结果表明,此AGP分布在细胞膜表面.

Keyword:

BY-2细胞 GPI锚 亚细胞定位 拟南芥 绿色荧光蛋白 阿拉伯半乳糖蛋白

Community:

  • [ 1 ] 福州大学生物科学与工程学院
  • [ 2 ] 俄亥俄大学环境与植物生物学系

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Source :

福州大学学报(自然科学版)

ISSN: 1000-2243

CN: 35-1337/N

Year: 2010

Issue: 05

Volume: 38

Page: 753-757

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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