Translated Title
Research on the Expression of Porcine Circovirus Capsid Protein in the Bacillus subtilis
Translated Abstract
The objective is to study the expression of porcine circovirus capsid protein in theBacillus subtilis. Based on 3 different plasmids,different types of recombinant vectors for porcine circovirus capsid protein gene(PCV2-ORF2)were constructed,and they were transformed into strain 168 and WB800 ofB. subtilis. The expression of the target protein was detected by SDS-PAGE and Western blot,and the mRNA expression of the target gene inB. subtilis was detected throughRT-qPCR. The original ORF2was difficult to be translated inB. subtilis expression system. After the codon optimization,truncated ORF2gene was successfully expressed as target protein in intracellular expression system ofB. subtilis. Thus,the optimization of the codons promoted the expression of the target gene.
Translated Keyword
Bacillus subtilis
codon optimization
porcine circovirus capsid protein
RT-qPCR
Access Number
WF:perioarticalswjstb201605018
