Background:The　advent　of　chromatographic　methods　has　significantly　revolutionized　studies　of　the　chemistry　of　many　fields　by　their　efficient　and　convenient　separation　of　molecules,thereof　the　feasibility　of　separating　intact　bacterial　cells　by　ion　exchange　chromatography　typical　for　macromolecular　study　was　investigated　in　this　paper.A　common　HPLC　system　coupled　with　a　light　scattering　detector　was　employed;TOYOPEARL　SuperQ-650C,　an　anion　exchanger,　was　used　as　the　column　media.