Translated Title
Optimization of Extraction Conditions of Total Flavonoids Compounds from Loguat Leaves by Plackett-Burman Design and Response Surface Analysis
Translated Abstract
Firstly we discussed the effect of each variable factor on the extraction rate of.Total Flavonoids Compounds(TFC)on the basis of single-factor experiment, then we screened the important variable factors with respect to their main effects on the extraction rate of TFC by Plackett-Burman(PB) design, and extraction temperature, ethanol concentration and solid-to-liquid ratio were identified as the most significant variables.Next we optimized the extraction conditions including these significant variables by Response Surface Analysis (Box-Behnken (BB) design) and obtained the optimum levels of these significant parameters which revealed these as follows:ethanol concentration of 65%, ultrasound temperature of 65 ℃, solid-liquid ratio of 1∶25.Under the optimum conditions, the predicted value of extraction rate of TFC is 10.27% and its experimental value is 10.29%.This result indicates that it is perfect to optimize of extraction conditions of TFC from Loguat Leaves by PB design and BB design.
Translated Keyword
Flavonoids compounds
Plackett Burman design
Response Surface Analysis
Ultrasound-assisted extraction
