Our　work　aims　to　explore　Raman　spectroscopy　(RS)　to　study　the　effects　of　total　protein　of　nasopharyngeal　carcinoma　(NPC)　cell　line　CNE2　treated　by　different　doses　of　X-ray　irradiation.　The　total　protein　is　extracted　from　the　cell　line　after　irradiation　with　no　incubation　(0　h)　and　with　incubation　for　72　h,　respectively.　Both　paired-samples　t　test　and　support　vector　machine　(SVM)　are　employed　for　statistical　analysis　of　Raman　spectrum.　The　results　show　that　several　Raman　bands　assigned　to　total　protein　can　be　detected;　and　the　X-ray　irradiation　have　various　effects　on　different　components　in　total　protein,　especially　for　the　dose　higher　than　6　Gy,　and　also　associated　with　the　incubation　time　after　irradiation.　The　differences　of　content　between　0　h　groups　and　72　h　groups　appear　at　bands　of　C-S　stretching,　C-H　stretching,　O=C-O　-　stretching　and　L-phenylalanine.　It　indicated　no　significant　diversification　on　structures　of　protein,　while　the　content　may　be　varied　during　irradiation.　Our　spectroscopic　results　reveal　that　RS　analysis　of　total　protein　of　cell　line　irradiated　by　X-ray,　in　conjunction　with　mathematical　statistical　model,　can　be　a　potential　method　to　explore　the　Raman　characterization　target　of　cell　radiosensitivity,　and　then　for　making　known　the　mechanism　of　radiosensitivity　of　tumor　further.　©　2012　-　IOS　Press　and　the　authors.　All　rights　reserved.