Study of pH measuring based on i-motif DNA conformation switch and UV-Vis absorption spectroscopy of gold nanoparticles - Details

author：

Zhong, J.-H. (Zhong, J.-H..) ^[1] | Guo, L.-Q. (Guo, L.-Q..) ^[2] | Wu, J.-M. (Wu, J.-M..) ^[3] | Chen, J.-F. (Chen, J.-F..) ^[4] | Chen, Z.-J. (Chen, Z.-J..) ^[5]

Indexed by：

Scopus PKU CSCD

Abstract：

A　fast,　sensitive,　colorimetric　method　for　the　detection　of　pH　based　on　the　differentiate　effect　of　gold　nanoparticles　to　the　configuration　of　DNA　was　developed　in　this　study.　The　UV-Vis　absorption　spectroscopy　of　the　i-motif　DNA-Au　NPs　system　has　been　investigated,　and　the　effect　of　the　concentration　of　salt　and　i-motif　DNA,　reaction　time　and　DNA　sequence　on　the　pH　response　of　the　system　have　been　also　optimized.　Under　the　optimum　conditions,　the　UV-Vis　absorption　spectroscopy　of　the　Au　NPs　is　changed　regularly　with　pH　in　the　range　of　5.3~7.0,　the　absorbance　at　520　nm　is　increased　gradually　while　at　700　nm　decreased.　Correspondingly,　the　color　of　the　Au　NPs　is　varied　from　violet　to　red.　The　pH　sensor　is　no　need　to　modification,　low　cost,　fast　and　can　be　carried　out　by　naked　eyes.　It　is　promising　to　use　in　monitoring　some　life　process　which　associated　with　pH　variation.

Keyword：

Colorimetric method; Gold nanoparticles (Au NPs); i-motif DNA; pH; UV-Vis absorption spectroscopy

Community：

[ 1 ] [Zhong, J.-H.]Longyan Entry-Exit Inspection and Quarantine Bureau, Longyan 364000, China
[ 2 ] [Zhong, J.-H.]State Key Testing Laboratory of Mineral, Longyan 364000, China
[ 3 ] [Zhong, J.-H.]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fuzhou University, Fuzhou 350108, China
[ 4 ] [Guo, L.-Q.]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fuzhou University, Fuzhou 350108, China
[ 5 ] [Wu, J.-M.]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fuzhou University, Fuzhou 350108, China
[ 6 ] [Chen, J.-F.]Longyan Entry-Exit Inspection and Quarantine Bureau, Longyan 364000, China
[ 7 ] [Chen, J.-F.]State Key Testing Laboratory of Mineral, Longyan 364000, China
[ 8 ] [Chen, J.-F.]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fuzhou University, Fuzhou 350108, China
[ 9 ] [Chen, Z.-J.]Longyan Entry-Exit Inspection and Quarantine Bureau, Longyan 364000, China
[ 10 ] [Chen, Z.-J.]State Key Testing Laboratory of Mineral, Longyan 364000, China

Reprint 's Address：

[Guo, L.-Q.]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fuzhou University, Fuzhou 350108, China

Email：

lqguo@fzu.edu.cn

Show more details

Related Keywords：

Source ：

Spectroscopy and Spectral Analysis

ISSN： 1000-0593

Year： 2012

Issue： 4

Volume： 32

Page： 1068-1071

0 . 2 9 3

JCR@2012

0 . 7 0 0

JCR@2023

JCR Journal Grade：4

CAS Journal Grade：4

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 2

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 0

Affiliated Colleges：

Get Fulltext

DOI Library Discovery Baidu Scholar Search SCOPUS

Type
Departments

All Years Choose Year From to