In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica - Details

author：

Peng, Z. (Peng, Z..) ^[1] | Liu, M. (Liu, M..) ^[2] | Fang, Z. (Fang, Z..) ^[3] | Zhang, Q. (Zhang, Q..) ^[4]

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Scopus

Abstract：

A　water-soluble　crude　polysaccharide　(WPS)　was　obtained　from　Laminaria　japonica　by　hot　water　extraction.　Three　major　polysaccharide　fractions　(WPS-1,　WPS-2　and　WPS-3)　were　purified　from　WPS　by　anion-exchange　chromatography.　Monosaccharide　components　analysis　indicated　that　galactose　was　the　predominant　monosaccharide　in　WPS　and　WPS-3,　accounting　for　56.25%　and　54.11%,　respectively.　And　fucose　was　the　predominant　monosaccharide　in　WPS-1　and　WPS-2,　accounting　for　46.91%　and　45.1%,　respectively.　Antioxidant　activity　tests　revealed　that　WPS-2　showed　significant　function　of　scavenging　hydroxyl　free　radical　and　WPS-1　exhibited　the　highest　inhibitory　effects　on　superoxide　radical.　Cytotoxicity　of　all　polysaccharide　fractions　was　evaluated　by　MTT　assay　and　Hoechst　33258　staining.　Results　showed　that　WPS-1　and　WPS-2　significantly　inhibited　the　growth　of　A375　cells　and　low　anti-proliferative　effects　of　WPS-2　on　vascular　smooth　muscle　cells　(VSMCs)　were　observed.　These　results　suggested　that　the　polysaccharide　fraction　of　WPS-2　might　be　explored　as　a　potential　safe　antioxidant　and　antitumor　agent.　©　2012　Elsevier　B.V.

Keyword：

Antioxidant activity; Cytotoxicity; Laminaria japonica polysaccharides

Community：

[ 1 ] [Peng, Z.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China
[ 2 ] [Peng, Z.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China
[ 3 ] [Liu, M.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China
[ 4 ] [Liu, M.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China
[ 5 ] [Fang, Z.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China
[ 6 ] [Zhang, Q.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China
[ 7 ] [Zhang, Q.]Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China

Reprint 's Address：

[Zhang, Q.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China

Email：

zhangqiq@126.com

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Source ：

International Journal of Biological Macromolecules

ISSN： 0141-8130

Year： 2012

Issue： 5

Volume： 50

Page： 1254-1259

2 . 5 9 6

JCR@2012

7 . 7 0 0

JCR@2023

JCR Journal Grade：3

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 42

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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