Using　L-tryptophan(L-Trp)　as　template　molecule　and　o-phenylenedia　mine　as　functional　monomer,　the　sensitive　molecularly　imprinted　polymer　films　was　prepared　on　the　surface　of　gold　electrode　by　in-situ　polymerization.　The　performance　of　the　imprinted　electrode　was　characterized　by　cyclic　voltammetry(CV),　differential　pulse　voltammetry(DPV)　and　electro-chemical　impedance　spectroscopy(EIS).　A　linear　relationship　between　oxidation　peak　current　and　concentration　of　L-Trp　was　obtained　over　a　range　from　1×10　-8　to　2×10　-7　mol/L　with　a　detection　limit　of　0.3×10　-8　mol/L.　From　the　selectivity　experiments,　the　imprinting　factor　of　L-Trp　imprinted　film　was　3.72　and　the　selectivity　factors　of　L-Trp　compared　to　interferents　were　all　larger　than　1.　Although　a　minor　interference　was　generated　by　L-Tyr　for　its　structure　similar　to　L-Trp,　the　selectivity　factor　also　reached　2.30.　The　results　indicated　that　the　imprinted　polymer　films　had　a　good　selectivity　for　L-Trp.　The　kinetics　of　recognition　revealed　a　two-step　process　of　the　imprinted　polymer　films　adsorbing　L-Trp,　which　contains　a　fast　binding　process　and　a　slow　adsorption　process.