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[期刊论文]

Enzyme-free and label-free ultrasensitive electrochemical detection of human immunodeficiency virus DNA in biological samples based on long-range self-assembled DNA nanostructures

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author:

Chen, X. (Chen, X..) [1] | Hong, C.-Y. (Hong, C.-Y..) [2] | Lin, Y.-H. (Lin, Y.-H..) [3] | Unfold

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Abstract:

Biosensors based on nanomaterials have been used for detection of various biological molecules with high sensitivity and selectivity. Herein, we developed a simple and ultrasensitive electrochemical DNA biosensor using long-range self-assembled DNA nanostructures as carriers for signal amplification, which can achieve an impressive detection limit of 5 aM human immunodeficiency virus (HIV) DNA even in complex biological samples. In this study, we designed two auxiliary probes. A cascade of hybridization events between the two auxiliary probes can lead to long-range self-assembly and form micrometer-long one-dimensional DNA nanostructures. In the presence of target DNA, each copy of the target can act as a trigger to connect a DNA nanostructure to a capture probe on the electrode surface. Then, a great amount of redox indicator [Ru(NH3)6]3+ can be electrostatically bound to the DNA nanostructures and eventually result in significantly amplified electrochemical signals. © 2012 American Chemical Society.

Community:

  • [ 1 ] [Chen, X.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China
  • [ 2 ] [Hong, C.-Y.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China
  • [ 3 ] [Lin, Y.-H.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China
  • [ 4 ] [Chen, J.-H.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China
  • [ 5 ] [Chen, J.-H.]Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China
  • [ 6 ] [Chen, G.-N.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China
  • [ 7 ] [Yang, H.-H.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China

Reprint 's Address:

  • [Chen, J.-H.]Key Lab of Analysis and Detection Technology for Food Safety, Fuzhou University, MOE, Fuzhou 350108, China

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Source :

Analytical Chemistry

ISSN: 0003-2700

Year: 2012

Issue: 19

Volume: 84

Page: 8277-8283

5 . 6 9 5

JCR@2012

6 . 8 0 0

JCR@2023

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

30 Days PV: 0

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