High-level expression of a sika deer (Cervus nippon) Cu/Zn superoxide dismutase in Pichia pastoris and its characterization - Details

author：

Li, R.-K. (Li, R.-K..) ^[1] | Fu, C.-L. (Fu, C.-L..) ^[2] | Chen, P. (Chen, P..) ^[3] | Ng, T.B. (Ng, T.B..) ^[4] | Ye, X.-Y. (Ye, X.-Y..) ^[5]

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Abstract：

Production　of　a　sika　deer　Cu/Zn-SOD　was　achieved　in　Pichia　pastoris　after　the　reconstituted　expression　vector　pPIC9K　was　transformed　into　the　strain　GS115.　By　employing　Saccharomyces　cerevisiae　secretion　signal　peptide　(α-factor)　under　the　regulation　of　the　methanol-inducible　promoter　of　the　gene　of　alcohol　oxidase　1　(AOX1),　sika　deer　Cu/Zn-SOD　with　a　molecular　mass　of　16.　kDa　was　expressed　while　recombinant　sika　deer　Cu/Zn-SOD　with　an　activity　of　3500.　U/mL　was　obtained　from　a　5.　L　bioreactor.　After　two　successive　steps　of　chromatography　on　DEAE-650.　C　and　Superdex75,　recombinant　sika　deer　Cu/Zn-SOD　was　obtained　with　13.8%　yield,　14.5-fold　purification,　and　a　specific　activity　of　3447.　U/mg.　Its　optimum　temperature　and　optimum　pH　were　40°C　and　7.0,　respectively.　©　2012.

Keyword：

Expression; Pichia pastoris; Purification; Sika deer; Superoxide dismutase

Community：

[ 1 ] [Li, R.-K.]College of Biological Science and Technology, Fuzhou University, China
[ 2 ] [Li, R.-K.]National Engineering Laboratory for High-efficiency Enzyme Expression, China
[ 3 ] [Fu, C.-L.]College of Biological Science and Technology, Fuzhou University, China
[ 4 ] [Chen, P.]National Engineering Laboratory for High-efficiency Enzyme Expression, China
[ 5 ] [Ng, T.B.]School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, NT Hong Kong, Hong Kong
[ 6 ] [Ye, X.-Y.]College of Biological Science and Technology, Fuzhou University, China
[ 7 ] [Ye, X.-Y.]National Engineering Laboratory for High-efficiency Enzyme Expression, China

Reprint 's Address：

[Ye, X.-Y.]College of Biological Science and Technology, Fuzhou University, Qi Shan Campus, 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, China

Email：

xiuyunye@fzu.edu.cn

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Source ：

Environmental Toxicology and Pharmacology

ISSN： 1382-6689

Year： 2013

Issue： 2

Volume： 35

Page： 185-192

1 . 8 6 2

JCR@2013

4 . 2 0 0

JCR@2023

JCR Journal Grade：2

CAS Journal Grade：4

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SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 0

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