Molecularly　imprinted　latex　membrane　(MILM)　is　prepared　by　core-shell　emulsion　technique　in　the　presence　of　a　template　molecule　(L-Tryptophan).　A　hard　inward　and　soft　outward　microstructure　latex　particle　is　designed　to　obtain　MILM　with　both　flexibility　and　impact　strength.　Molecularly　imprinted　layer　with　high　crosslinking　degree　is　grafted　on　the　surface　of　core-shell　latex　particles.　NaCl,　glucose,　urea,　polyethylene　glycol,　M　w　300,　etc.,　are　added　during　the　film-forming　process　to　produce　porous　microstructure　in　MILM.　Fourier　transform　infrared　spectroscopy　(FTIR)　and　Scatchard　analysis　are　used　to　investigate　the　interaction　between　L-Tryptophan　and　MILM　and　the　binding　ability　of　the　resultant　MILM,　respectively.　The　functional　binding　and　separation　performances　in　aqueous　medium　towards　template　are　carried　out.　The　results　reveal　that　the　content　and　type　of　porogen　and　the　shell　composition　have　significant　effects　on　adsorption　capacity　and　separation　ability.　MILM　with　glucose　as　porogen　shows　high　recognition　towards　the　template　with　adsorption　separation　factor　reaching　9.06.　©　2012　Wiley　Periodicals,　Inc.