This　study　aimed　to　study　the　effects　of　microwave　radiation　on　the　nasopharyngeal　carcinoma　cell　line　CNE2　by　Raman　spectroscopy.　The　cells　were　separated　into　a　control　group　and　radiated　groups　with　radiation　times　of　2,　5,　10,　and　25　min,　respectively.　Both　principal　components　analysis　and　support　vector　machine　were　employed　for　statistical　analysis　of　Raman　spectra.　The　results　show　that　the　relative　content　of　C-H　deformation　and　amide　I　begin　to　change　when　the　radiation　time　is　over　10　min,　and　principal　components　analysis　further　confirms　there　are　significant　differences　after　10　min　of　radiation.　Moreover,　support　vector　machine　is　simultaneously　used　to　classify　radiated　samples　from　control　samples.　The　classification　accuracy　is　low　until　the　radiation　time　reaches　over　10　min.　In　conclusion,　this　study　reveals　the　Raman　spectral　characteristics　of　CNE2　under　different　microwave　radiation　exposure　timesand　demonstrates　Raman　spectroscopy　can　be　a　potential　method　to　explore　cellular　characterization　after　radiation.　The　final　results　may　help　in　elucidating　the　mechanism　by　which　microwave　radiation　interacts　with　tumor　cells.　©　2013　Published　by　NRC　Research　Press.