Via　covalent　coupling　of　epoxy　group　＞　an　open　tubular　column　covalently　modified　with　carboxymethyl　chitosan　(CMC)　as　stationary　phase　was　prepared.　The　parameters　of　column　pretreatment　＞　silanization　and　chemically　bonding　of　CMC　were　optimized.　The　morphology　of　inner　wall　of　the　open　tubular　column　was　observed　by　scanning　electron　microscope　(SEM/　＞　which　showed　that　the　inner　wall　of　the　prepared　column　was　evenly　coated　with　a　polymer　film.　Either　positive　or　negative　electroosmotic　flow　(EOF)　could　be　achieved　by　varying　the　pH　values　of　running　buffer.　Good　reproducibility　was　obtained　with　the　relative　standard　deviations　(RSDs)　of　EOF　less　than　0.　8%　for　run-to-run　\n　=6/　＞　less　than　3.　5%　for　day-to-day　\n　=3)　＞　less　than　4.　3%　for　column-to-column　(TO　=3)　and　less　than　6.　1%　for　batch-to-batch　(TO　=3).　Nucleotides　(AMP＞　GMP＞　CMP　and　UMP)　were　analyzed　on　the　open　tubular　column　modified　with　CMC＞　with　high　column　efficiencies　ranging　from　36　000　to　182　000　plates/m.　The　results　indicate　that　the　developed　method　is　effective;　easy　and　stable.