Silver nanowire-graphene hybrid nanocomposites as label for sensitive electrochemical immunoassay of alpha-fetoprotein - Details

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Abstract：

A　facile　and　sensitive　mediator-free　electrochemical　immunoassay　was　developed　for　determination　of　alpha-fetoprotein　(AFP)　in　human　serum　with　a　sandwich-type　mode　by　using　silver　nanowire-graphene　hybrid　nanocomposites　(AgNW-GPs)　as　label.　One-pot　hydrothermal　method　was　used　for　the　synthesis　of　the　AgNW-GPs　with　the　aid　of　trisodium　citrate.　The　as-prepared　AgNW-GP　was　not　only　utilized　for　the　label　of　horseradish　peroxidase-conjugated　anti-AFP　(HRP-anti-AFP)　with　highly　loading　capacity,　but　also　displayed　good　electrochemical　behaviors.　Compared　with　the　conventional　HRP-anti-AFP-based　electrochemical　immunoassay,　the　sensitivity　of　the　proposed　immunoassay　by　using　the　synthesized　nanolabels　was　greatly　improved.　Under　optimal　conditions,　the　electrochemical　immunosensor　exhibited　a　wide　linear　range　of　0.05-400　ng/mL　with　a　low　detection　limit　(LOD)　of　5　pg/mL　AFP　(at　3sB).　Intra-　and　inter-assay　coefficients　of　variation　were　below　10%　and　8.5%,　respectively.　The　selectivity　and　stability　of　the　immunosensor　were　acceptable.　In　addition,　the　methodology　was　also　validated　by　assaying　16　positive　serum　samples　and　7　negative　serum　samples,　receiving　a　good　correlation　with　the　results　obtained　from　the　referenced　electrochemiluminescence　method.　©　2011　Elsevier　Ltd.

Keyword：

Alpha-fetoprotein; Biomarker; Electrochemical immunosensor; Graphene nanosheet; Silver nanowire

Community：

[ 1 ] [Tang, J.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 2 ] [Tang, J.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 3 ] [Tang, D.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 4 ] [Tang, D.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 5 ] [Su, B.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 6 ] [Su, B.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 7 ] [Li, Q.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 8 ] [Li, Q.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 9 ] [Li, Q.]College of Pharmaceutical Sciences, Zunyi Medical College, Zunyi 563003, China
[ 10 ] [Qiu, B.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 11 ] [Qiu, B.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 12 ] [Chen, G.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
[ 13 ] [Chen, G.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China

Reprint 's Address：

[Tang, D.]Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350108, China

Email：

dianping.tang@fzu.edu.cn

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Source ：

Electrochimica Acta

ISSN： 0013-4686

Year： 2011

Issue： 24

Volume： 56

Page： 8168-8175

3 . 8 3 2

JCR@2011

5 . 5 0 0

JCR@2023

JCR Journal Grade：2

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 32

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 7

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