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author:

Lin, J. (Lin, J..) [1] | Gao, Z. (Gao, Z..) [2] | Liu, S. (Liu, S..) [3] | Rao, P. (Rao, P..) [4]

Indexed by:

Scopus PKU CSCD

Abstract:

Ralstonia solanacearum is one of the most important plant pathogens as it causes great economic losses worldwide. In this work, high performance liquid chromatography coupled to a multi-angle laser light scattering detector (HPLC7 MALLS) was applied to investigate the adsorption characteristics of wild type strain 79# and avirulent mutant RS52' of R. solanacearum onto strong anion exchange resins (SuperQ-650C). The results showed that the two strains differed greatly in their chromatographic traces and adsorbility. In order to further confirm these results, batch adsorption experiments were carried out to determine the adsorption speed and amount. The adsorption amount of strain 79# reached equilibrium at 60 min and the maximum value was 1.09×10 9 cfu/g(dry resin), whereas the adsorption amount of strain RS52' reached equilibrium at 120 min and the maximum value was 2.10×10 9 cfu/g(dry resin). Furthermore, Langmuir and Freundlich adsorption isotherm models were employed to study the interactive behaviors between bacteria and resins. The results indicated that the adsorbility of strain RS52' was stronger than that of strain 79#, which was in good agreement with the results of the batch adsorption experiments. Additionally, the experimental data of R. solanacearum adsorption onto SuperQ-650C resins, especially for the wild type strain 79#, showed a better fit with Freundlich isotherm model than with Langmuir model.

Keyword:

Adsorption characteristics; Avirulent mutant; Chromatographic traces; Ralstonia solanacearum; Wild type

Community:

  • [ 1 ] [Lin, J.]College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China
  • [ 2 ] [Gao, Z.]College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China
  • [ 3 ] [Liu, S.]College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China
  • [ 4 ] [Rao, P.]College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China

Reprint 's Address:

  • 林娟

    [Lin, J.]College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China

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Source :

Chinese Journal of Applied and Environmental Biology

ISSN: 1006-687X

Year: 2010

Issue: 4

Volume: 16

Page: 499-503

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 2

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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