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Abstract:
Ralstonia solanacearum is one of the most important plant pathogens as it causes great economic losses worldwide. In this work, high performance liquid chromatography coupled to a multi-angle laser light scattering detector (HPLC7 MALLS) was applied to investigate the adsorption characteristics of wild type strain 79# and avirulent mutant RS52' of R. solanacearum onto strong anion exchange resins (SuperQ-650C). The results showed that the two strains differed greatly in their chromatographic traces and adsorbility. In order to further confirm these results, batch adsorption experiments were carried out to determine the adsorption speed and amount. The adsorption amount of strain 79# reached equilibrium at 60 min and the maximum value was 1.09×10 9 cfu/g(dry resin), whereas the adsorption amount of strain RS52' reached equilibrium at 120 min and the maximum value was 2.10×10 9 cfu/g(dry resin). Furthermore, Langmuir and Freundlich adsorption isotherm models were employed to study the interactive behaviors between bacteria and resins. The results indicated that the adsorbility of strain RS52' was stronger than that of strain 79#, which was in good agreement with the results of the batch adsorption experiments. Additionally, the experimental data of R. solanacearum adsorption onto SuperQ-650C resins, especially for the wild type strain 79#, showed a better fit with Freundlich isotherm model than with Langmuir model.
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Chinese Journal of Applied and Environmental Biology
ISSN: 1006-687X
Year: 2010
Issue: 4
Volume: 16
Page: 499-503
Cited Count:
SCOPUS Cited Count: 2
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 1
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