The　electrochemical　behaviors　occurring　in　the　interface　of　chalcopyrite-carbon　paste　and　electrolyte　were　evaluated　by　cyclic　voltammetry　method　in　the　presence　and　absence　of　bacterial　strains.　The　effects　of　temperature,　limit　switching　cathodic　potential,　Fe2+　and　Cu2+　irons　on　the　cyclic　voltammetry　spectra　were　examined.　The　results　showed　that　an　oxidation　peak　of　polysulfide　formation　appeared　at　0.45　V　in　the　bioleaching　of　low　grade　chalcopyrite.　At　-0.25　and　-0.43　V,　reduction　peaks　of　the　formation　of　bornite　and　chalcocite　appeared　respectively.　The　higher　the　temperature,　the　faster　the　accelerating　rate　of　chalcopyrite.　The　value　of　oxidation　peak　current　at　65°C　was　twice　as　that　of　at　35°C.　The　addition　of　Fe2+　and/or　Cu2+　ions　in　solution　also　enforced　the　oxidation　of　chalcopyrite.