A　method　for　the　determination　of　six　perfluorinated　organic　compounds　(　PFCs　)　in　feed　has　been　developed.　It　is　based　on　polyamide　solid-phase　extraction　(　SPE　)　together　with　ultra　performance　liquid　chromatography-tandem　mass　spectrometry　(　UPLC-MS/MS　).　The　sample　was　extracted　by　acidified　acetonitrile.　The　extraction　solution　was　enriched　by　a　poly-amide　SPE　cartridge　under　acidic　condition,　and　cleaned-up　using　methanol,　eluted　by　5%　(v/v)　ammonia/methanol　solvent　and　determined　by　UPLC-MS/MS.　The　UPLC　separation　was　carried　out　on　an　Acquity　BEH　C　18　column　(　100　mm×2.　1　mm,　1.　7　μm　).　The　mobile　phases　were　5　mmol/L　ammonium　acetate　and　acetonitrile　with　a　gradient　elution.　Under　the　optimal　conditions,　the　PFCs　were　analyzed　under　multiple　reaction　monitoring　(　MRM　)　mode　with　neg-ative　electrospray　ionization.　The　isotope　internal　standard　method　was　used　to　determine　the　six　PFCs,　and　improve　the　quantitative　accuracy.　All　of　the　target　compounds　exhibited　good　linearity　(r>0.　995)　over　a　concentration　range　of　0.　5-25　μg/L.　The　detection　limits　of　the　six　PFCs　were　all　smaller　than　0.　1　μg/kg.　The　mean　recoveries　of　the　six　PFCs　were　in　the　range　of　94.　2%　to　108.　9%　with　the　relative　standard　deviations　(　RSDs)　of　1.　8%-8.　6%　(n　=　6).　The　method　for　the　determination　of　PFCs　in　feed　is　low-cost,　favorable　effect　and　suitable　for　the　detection　of　complex　matrix　samples.