Magnetic beads based colorimetric detection of mercuric ion - Details

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Abstract：

A　simple　and　sensitive　DNAzyme-based　colorimetric　sensor　for　detecting　Hg2+　based　on　T-Hg2+-T　base　pair　and　magnetic　beads　(MBs)　is　proposed.　In　the　presence　of　Hg2+,　capture　probes　(CPs)　immobilized　on　MBs　are　hybridized　with　the　signal　probes　(SPs)　consisting　of　G-quadruplex　sequences　with　the　help　of　T-Hg2+-T　base　pairs.　The　SPs　are　separated　by　MBs　and　used　to　bind　hemin　for　the　formation　of　catalytically　active　hemin/G-quadruplex　DNAzymes,　which　catalyzes　the　H2O　2-mediated　oxidation　of　ABTS　to　produce　a　blue-green-colored　product.　A　linear　relationship　between　the　absorbance　and　Hg2+　concentration　is　observed　over　a　range　of　10-200　nM　with　a　limit　of　detection　5　nM.　Taking　advantage　of　MBs　separation/collection　process,　the　sensing　system　can　be　regenerated　by　using　EDTA　as　the　chelator.　©　2013　Elsevier　B.V.　All　rights　reserved.

Keyword：

DNAzyme; Magnetic beads; Mercury; T-Hg2+-T

Community：

[ 1 ] [Huang, X.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 2 ] [Hao, Y.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 3 ] [Wu, H.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 4 ] [Guo, Q.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 5 ] [Guo, L.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 6 ] [Wang, J.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 7 ] [Zhong, L.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 8 ] [Lin, T.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 9 ] [Fu, F.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China
[ 10 ] [Chen, G.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China

Reprint 's Address：

[Guo, L.]Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, China

Email：

lqguo@fzu.edu.cn

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Source ：

Sensors and Actuators, B: Chemical

ISSN： 0925-4005

Year： 2014

Volume： 191

Page： 600-604

4 . 0 9 7

JCR@2014

6 . 3 9 3

JCR@2018

JCR Journal Grade：1

CAS Journal Grade：1

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WoS CC Cited Count：

SCOPUS Cited Count： 16

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 0

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