The　retentate　from　membrane　bioreactor　(MBR)　and　nanofiltration　(NF)　combined　process　for　incineration　leachate　is　difficult　to　be　treated　due　to　high　content　of　refractory　organic　matter　and　inorganic　salt.　Taking　into　consideration　of　the　components　of　refractory　organic　matter　in　the　rtentate　are　mainly　humic　substances　(HS),　the　pilot-scale　experiments　were　conducted　to　investigate　separation　and　recovery　of　HS　from　MBR-NF　retentate　of　incineration　leachate　by　ultrafiltration　(UF).　The　results　showed　that　the　UF　could　effectively　separate　and　purity　organic　matter　in　the　MBR-NF　retentate　from　inorganic　ions.　The　data　indicate　that　separation　factor　(SF)　is　strongly　correlated　with　concentration　factor　(CF).　The　content　of　HS　in　the　recovering　sample　was　more　than　36　g/L,　and　the　concentrations　of　heavy　metals　were　lower　than　the　content-limit　of　related　standard.　This　study　confirmed　the　feasibility　of　recovering　HS　from　MBR-NF　retentate　of　incineration　leachate　by　UF.