An aptamer-based biosensor for sensitive thrombin detection with phthalocyanine@SiO 2 mesoporous nanoparticles - Details

author：

Jiang, Z. (Jiang, Z..) ^[1] | Yang, T. (Yang, T..) ^[2] | Liu, M. (Liu, M..) ^[3] | Hu, Y. (Hu, Y..) ^[4] | Wang, J. (Wang, J..) ^[5]

Indexed by：

Scopus

Abstract：

Silica　nanoparticles,　with　entrapped　hydrophobic　photosensitizer　tetra-α-(2,　4-di-tert-butylphenoxy)-phthalocyaninato　zinc　(ZnPc(OAr)　4　),　were　synthesized　by　hydrolyzing　triethoxyvinylsilane　(TEVS)　and　3-amino　propyl　triethoxysilane　(APTES).　The　as-prepared　nanoparticles,　which　were　highly　monodispersed　spheres　(about　100nm),　exhibited　strong　Q-band　absorption　of　ZnPc(OAr)　4　centered　at　701nm.　The　aqueous　solubility　of　ZnPc(OAr)4　encapsulated　in　silica　nanoparticles　was　obviously　improved.　The　nanoparticles　efficiently　generated　singlet　oxygen　(　1　O　2　)　both　in　organic　and　aqueous　solutions　after　being　irradiated　at　suitable　wavelength.　The　resulting　1　O　2　then　initiated　chemiluminescence　(CL)　by　reacting　with　a　methyl　cypridina　luciferin　analog　(MCLA).　Based　on　the　photoinduced　CL,　a　sensitive　aptamer-based　sandwich-type　sensor　was　presented　to　detect　human　thrombin.　Thrombin　was　first　attached　to　Pc@SiO　2　via　secondary　aptamer,　and　then　they　were　collected,　for　CL　determination,　by　beads　with　primary　aptamer.　The　proposed　approach,　which　was　highly　selective　with　a　low　detection　limit　of　80pmol/L,　minimized　the　nonspecific　adsorption.　Such　an　aptamer-based　biosensor　is　feasible　in　screening　biomarkers　in　complex　matrices　at　ultratrace　levels.　©　2013　Elsevier　B.V.

Keyword：

Chemiluminescence; Silica nanoparticle; Tetra-α-(24-di-tert-butylphenoxy)-phthalocyaninato zinc; Thrombin

Community：

[ 1 ] [Jiang, Z.]Institute of Research on Functional Materials, Cancer Metastasis Alert and Prevention Center, Fuzhou University, Fuzhou 350108, China
[ 2 ] [Jiang, Z.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China
[ 3 ] [Yang, T.]Institute of Research on Functional Materials, Cancer Metastasis Alert and Prevention Center, Fuzhou University, Fuzhou 350108, China
[ 4 ] [Yang, T.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China
[ 5 ] [Liu, M.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350108, China
[ 6 ] [Liu, M.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China
[ 7 ] [Hu, Y.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350108, China
[ 8 ] [Hu, Y.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China
[ 9 ] [Wang, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350108, China
[ 10 ] [Wang, J.]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China

Reprint 's Address：

[Wang, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou 350108, China

Email：

jwang@fzu.edu.cn

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Source ：

Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2014

Volume： 53

Page： 340-345

6 . 4 0 9

JCR@2014

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：268

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 30

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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