A　new　biosensing　platform　based　on　electrospun　carbon　nanotubes　nanofibers　(CNTs@PNFs)　composite,　which　enabled　strong　electrochemiluminescent　emission　of　peroxydisulfate,　was　firstly　developed　for　immunoassay　with　favorable　analytical　performances,　and　then　was　utilized　to　evaluate　the　interaction　between　antibody　and　antigen　in　vitro.　Moreover,　the　obvious　ECL　image　of　peroxydisulfate　on　the　prepared　sensing　platform　was　firstly　recorded　in　this　report.　In　order　to　expand　the　application　of　peroxydisulfate　ECL,　the　specific　recognization　biomolecules,　α-fetoprotein　(AFP)　antibody　was　bound　to　the　functionalized　film　via　electrostatic　interaction　for　fabricating　label-free　ECL　immunosensor　to　detect　α-AFP.　Based　on　the　ECL　change　resulting　from　the　specific　immunoreaction　between　antigen　and　antibody,　the　quantitative　analysis　for　AFP　with　wide　dynamic　response　in　the　range　from　0.1pgmL-1　to　160ngmL-1　was　realized.　And　the　limit　of　detection　was　estimated　to　be　0.09pgmL-1.　Therefore,　the　flexible　sensing　platform　not　only　acted　as　the　sensitized　sensing　element,　but　also　offered　a　suitable　carrier　for　immobilization　of　biological　recognition　elements　with　low-toxicity　and　eco-friendliness,　which　opened　a　promising　approach　to　developing　further　electrospun　nanofiber　based　amplified　ECL　biosensor　with　favorable　analytical　performances.　©　2014　Elsevier　B.V.