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[期刊论文]

Screening of Marine Microorganisms Produced α-Amylase and It's Enzymatic Properties

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author:

Lin, M. (Lin, M..) [1] | Wang, G. (Wang, G..) [2] | Ye, X. (Ye, X..) [3] | Unfold

Indexed by:

Scopus PKU CSCD

Abstract:

Strain 825 with α-amylase activity was isolated from marine samples of Pingtan sea and Tai wan strait and identified as Bacillus sp. by 16S rDNA sequence analysis. Amylase-producing medium of strain 825 was optimized, and the optimum conditions were as follow: soluble starch 2.0%, yeast extract 0.5%, peptone 1.5%, initial pH7.0, fermentation period 60 h. Under these conditions, α-amylase activity increased from 46.55 U/mL to 77.44 U/mL, 66.36% higher than before. Furthermore, the α-amylase properties were analyzed and the results were obtained. The optimal reaction temperature of α-amylase was 70℃, and more than 90% of its maximum activity was maintained after incubation for 1 h under 30-50℃. The optimal reaction pH was 7.0, and more than 70% activity was retained after incubation for 1 h in the pH 5.0-9.0. It indicated that this α-amylase had a wide pH tolerance range. © 2017, Editorial Office of Journal of CIFST. All right reserved.

Keyword:

Enzymatic properties; Isolation and identification; Marine microorganism; Optimization of fermentation conditions; α-amylase

Community:

  • [ 1 ] [Lin, M.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
  • [ 2 ] [Wang, G.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
  • [ 3 ] [Wang, G.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China
  • [ 4 ] [Ye, X.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
  • [ 5 ] [Ye, X.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China
  • [ 6 ] [Lin, J.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
  • [ 7 ] [Lin, J.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China

Reprint 's Address:

  • [Lin, J.]College of Biological Science and Technology, Fuzhou UniversityChina

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Source :

Journal of Chinese Institute of Food Science and Technology

ISSN: 1009-7848

Year: 2017

Issue: 2

Volume: 17

Page: 77-84

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 2

30 Days PV: 2

Affiliated Colleges:

操作日志

管理员  2025-04-09 22:14:19  更新被引

管理员  2024-08-23 17:53:17  更新被引

管理员  2024-07-30 05:55:20  更新被引

管理员  2020-11-20 11:12:01  创建

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