Studies on Agarase-producing Conditions of Marine Vibrio sp. Ag-1 - Details

author：

Zhang, J. (Zhang, J..) ^[1] | Han, Y. (Han, Y..) ^[2] | Wang, G. (Wang, G..) ^[3] | Ye, X. (Ye, X..) ^[4] | Lin, J. (Lin, J..) ^[5]

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Abstract：

A　marine　agarase-producing　strain　(Vibrio　sp.)Ag-1　was　isolated　from　asparagus　and　its　medium　compositions　and　fermentation　conditions　for　agarase　production　were　studied.　The　optimal　agarase-producing　conditions　were　obtained:　agar　0.2%,　peptone　0.3%,　yeast　extract　0.8%,　NaCl　4.0%,　MgSO4　0.6%,　CaCl2　0.2%,　initial　pH　of　medium　6.5,　inoculation　volume　1%　(v/v),　shaking　speed　240　r/min,　medium　volume　50　mL/250mL,　temperature　33℃,　fermentation　time　24　h.　Fermenting　under　these　conditions,　agarase　activity　reached　45.51　U/mL,　about　5.5　times　higher　than　before　optimization　(7.0　U/mL).　©　2017,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.

Keyword：

Agarase; Optimization of agarase-producing conditions; Optimization of medium; Vibrio sp.

Community：

[ 1 ] [Zhang, J.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, China
[ 2 ] [Han, Y.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, China
[ 3 ] [Wang, G.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, China
[ 4 ] [Ye, X.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, China
[ 5 ] [Lin, J.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, China

Reprint 's Address：

[Lin, J.]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou UniversityChina

Email：

ljuan@fzu.edu.cn

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Source ：

Journal of Chinese Institute of Food Science and Technology

ISSN： 1009-7848

Year： 2017

Issue： 3

Volume： 17

Page： 90-95

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ESI Highly Cited Papers on the List： 0 Unfold All

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