Isolation and Identification of Chitinase-producing Strains and Studies on the Enzymatic Properties of Chitinase - Details

author：

Lian, W. (Lian, W..) ^[1] | Lin, J. (Lin, J..) ^[2] | Wang, G. (Wang, G..) ^[3] | Ye, X. (Ye, X..) ^[4] | Su, B. (Su, B..) ^[5]

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Abstract：

A　chitinase-producing　strain　B120　was　isolated　from　Fujian　soil　and　identified　as　Bacillus　cereus　by　16S　rDNA　sequence　analysis.　The　chitinase-producing　media　were　optimized　and　the　optimal　medium　formulations　were　ob　tained　and　as　follow:　lactose　3.5%,　peptone　1.0%,　powdered　chitin　0.2%,　K2HPO4　0.07%,　KH2PO4　0.03%,　MgSO4　0.05%,　FeSO4-H20　0.002%,　NaCl　0.5%,　pH　6.0.　Fermenting　under　these　conditions　for　84　h,　chitinase　activity　could　reach　603　U/L,　5.93　times　higher　than　before　optimization　(87　U/L).　Furthermore,　the　chitinase　properties　were　studied.　The　results　showed　that　the　optimum　reaction　pH　and　temperature　were　8.0　and　50℃,　respectively.　This　enzyme　was　stable　under　30℃　and　pH　7.0-10.0,　and　more　than　74%　of　relative　activity　was　retained　after　incubation　for　1　h.　It　was　a　kind　of　mesothermal　and　alkaline　chitinases.　©　2017,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.

Keyword：

Bacillus cereus; Chitinase; Enzymatic properties; Identification; Optimization of medium; Screening

Community：

[ 1 ] [Lian, W.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
[ 2 ] [Lin, J.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
[ 3 ] [Lin, J.]National Engineering Laboratory for High Efficient Enzyme Expression, Fuzhou, 350002, China
[ 4 ] [Wang, G.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
[ 5 ] [Wang, G.]National Engineering Laboratory for High Efficient Enzyme Expression, Fuzhou, 350002, China
[ 6 ] [Ye, X.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China
[ 7 ] [Ye, X.]National Engineering Laboratory for High Efficient Enzyme Expression, Fuzhou, 350002, China
[ 8 ] [Su, B.]College of Biological Science and Technology, Fuzhou University, Fuzhou, 350116, China

Reprint 's Address：

[Lin, J.]College of Biological Science and Technology, Fuzhou UniversityChina

Email：

ljuan@fzu.edu.cn

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Source ：

Journal of Chinese Institute of Food Science and Technology

ISSN： 1009-7848

Year： 2017

Issue： 3

Volume： 17

Page： 82-89

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ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 3

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