We　propose　an　effective　and　efficient　method　that　reduces　the　crosstalk　level　in　autostereoscopic　light-emitting　diode　(LED)　displays　by　optimizing　the　pixel　arrangement　of　the　associated　two-dimensional　(2-D)　LED　panel.　In　the　proposed　method,　first　a　series　of　parallax　barrier　patterns,　based　on　predesignated　LED　packaging　units,　are　designed　and　simulated　by　sequentially　regulating　the　width　of　black　stripes　on　the　2-D　LED　panel.　This　design　principle　removes　the　black　stripes　from　conventional　autostereoscopic　LED　display　pixels　for　optimal　parallax　barrier　calculation.　Furthermore,　the　mathematical　relationship　between　average　crosstalk　level　and　visual　flux　density　is　obtained　from　the　simulation.　A　mathematical　fitting　method　that　includes　a　cubic　fitting　function　is　finally　applied　to　achieve　proper　pixel　arrangement　in　the　2-D　LED　panel.　The　simulation　results　obtained　for　a　dual　viewpoint　autostereoscopic　LED　display　system　indicate　that　the　most　suitable　value　for　the　width　of　the　black　stripes　is　within　the　range　of　3.17604　to　3.34277　mm,　with　a　light-emitting　pixel　width　of　2　mm.　This　method　can　effectively　guide　the　2-D　LED　panel＇s　design　and　result　in　high　performance　autostereoscopic　three-dimensional　LED　displays,　which　will　have　broad　application　prospects　in　the　near　future.　©　2017　Society　of　Photo-Optical　Instrumentation　Engineers　(SPIE).