Backbone and side-chain resonance assignments for the tmRNA-binding protein, SmpB, from Mycobacterium tuberculosis - Details

author：

Yang, J. (Yang, J..) ^[1] | Liu, Y. (Liu, Y..) ^[2] | Liu, Z. (Liu, Z..) ^[3] | Meng, C. (Meng, C..) ^[4] | Lin, D. (Lin, D..) ^[5]

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Abstract：

Small　protein　B　(SmpB)　is　an　essential　molecule　in　trans-translation　which　is　a　universal　biological　pathway　for　protein　synthesis　in　bacteria.　Trans-translation　can　release　stalled　ribosomes　from　defective　mRNAs　and　target　tag-protein　fragments　for　degradation,　and　then　restart　protein　synthesis.　The　SmpB-tmRNA　complex　coordinating　with　other　components　of　the　trans-translation　system,　plays　vital　roles　in　Mycobacterium　tuberculosis　under　both　stress　conditions　and　non-replicating　conditions.　Thus,　elucidation　of　molecular　details　and　dynamic　properties　of　the　SmpB-tmRNA　interaction　is　a　crucial　step　towards　effectively　blocking　trans-translation　process　to　shorten　the　duration　of　tuberculosis　treatment.　Here,　we　report　resonance　assignments　for　1H,　13C　and　15N　of　M.　tuberculosis　SmpB　(MtSmpB,　spanning　residues　4–133)　protein　determined　by　a　suite　of 2D/3D　heteronuclear　NMR　experiments　along　with　predicted　the　secondary　structure.　©　2017,　Springer　Science+Business　Media　Dordrecht.

Keyword：

MtSmpB; NMR resonance assignment; Secondary structure prediction; Trans-translation; Tuberculosis

Community：

[ 1 ] [Yang, J.]Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350108, China
[ 2 ] [Liu, Y.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 3 ] [Liu, Z.]Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350108, China
[ 4 ] [Meng, C.]Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350108, China
[ 5 ] [Lin, D.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China

Reprint 's Address：

[Lin, D.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen UniversityChina

Email：

dhlin@xmu.edu.cn

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Source ：

Biomolecular NMR Assignments

ISSN： 1874-2718

Year： 2017

Issue： 2

Volume： 11

Page： 175-179

0 . 5 9 3

JCR@2017

0 . 8 0 0

JCR@2023

ESI HC Threshold：231

JCR Journal Grade：4

CAS Journal Grade：4

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WoS CC Cited Count： 0

SCOPUS Cited Count： 1

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 1

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