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Abstract:
A class of 0-dimensional/2-dimensional (0D/2D) nanoheterostructures based on carbon quantum dots (CQDs) and graphitic carbon nitride (g-C 3 N 4 ) was designed as the signal-generation tags for the sensitive photoelectrochemical (PEC) immunoassay of prostate-specific antigen (PSA) coupling with the copper nanoclusters (CuNCs). Combination of CQDs with g-C 3 N 4 promoted the photoexcited electron/hole separation and largely increased the photocurrents of the nanoheterostructures. Initially, a sandwich-type immunoreaction was carried out on monoclonal anti-PSA antibody-coated microplate by using PSA aptamer linked with CuNCs as the tracer. Accompanying the immunocomplex, the carried CuNCs were dissolved under acidic conditions. The as-released copper ions from the CuNCs could be captured onto the CQDs/g-C 3 N 4 nanoheterostructures via the amino-group on the CQD surface as well as the -NH x (x = 1, 2, 3) of g-C 3 N 4 nanosheets. The strong coordination of the Lewis basic sites on the CQDs/g-C 3 N 4 with Cu 2+ decreased the photocurrent of the nanoheterostructures. Under optimal conditions, CQDs/g-C 3 N 4 nanoheterostructures displayed good photocurrent responses for the detection of PSA within the dynamic linear range of 0.02-100 ng mL -1 and a limit of detection (LOD) of 5.0 pg mL -1 . This method was also evaluated for quantitative screening of human PSA serum specimens by using the referenced electrochemiluminescent enzyme-linked immunoassay (ECL-ELIA) and gave good matched results between two methods. Additionally, this system was beneficial to explore the charge-separation and photoinduced electron transfer mechanism in the photoelectrochemical sensing protocols. © 2017 American Chemical Society.
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ACS Applied Materials and Interfaces
ISSN: 1944-8244
Year: 2017
Issue: 44
Volume: 9
Page: 38336-38343
8 . 0 9 7
JCR@2017
8 . 5 0 0
JCR@2023
ESI HC Threshold:306
JCR Journal Grade:1
CAS Journal Grade:2
Cited Count:
SCOPUS Cited Count: 143
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 3
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