Identification and Characterization of Antibacterial Metabolites and Optimization of Cultural Conditions for Bacillus subtilis [枯草芽孢杆菌抑菌活性物质鉴定, 抑菌特性及发酵条件优化] - Details

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Abstract：

The　active　metabolites　of　Bacillus　subtilis　BS08　against　bacterium　purified　from　fish　were　identified　by　Infrared　Spectroscopy　and　HPLC-MS.　Liquid-state　fermentation　by　BS08　was　optimized　for　the　maximum　antibacterial　activity　by　response　surface　methodology　(RSM).　The　active　metabolites　were　extracted　by　acid　and　alcohol,　purified　by　TLC　and　identified　by　Infrared　Spectroscopy　and　HPLC-MS.　The　analysis　revealed　the　active　metabolites　were　families　of　lipopeptides,　such　as　surfactin　and　iturin　with　molecular　mass　995-1　350,　thermal　and　pH　stability,　widely　antimicrobial　spectrum.　Response　surface　methodology　(RSM)　was　employed　to　optimize　the　cultural　conditions　of　BS08　for　the　enhancement　of　lipopeptides.　BS08　with　7%　inoculum　was　cultivated　at　38　℃,　200　r/min,　pH　8.2　for　24　h　in　containing　yeast　extract　14　g/L,　glucose　14　g/L,　Mg　SO41　g/L.　The　yield　of　lipopeptides　reached　450　mg/L.　©　2017,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.

Keyword：

Antibacterial; Bacillus subtilis BS08; Lipopeptides; Optimization

Community：

[ 1 ] [Zhang, W.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China
[ 2 ] [Bian, D.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China
[ 3 ] [Shen, Y.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China
[ 4 ] [Wang, W.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China
[ 5 ] [Liu, Z.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China
[ 6 ] [Ni, L.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China

Reprint 's Address：

[Ni, L.]Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou UniversityChina

Email：

nili@fzu.edu.cn

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Source ：

Journal of Chinese Institute of Food Science and Technology

ISSN： 1009-7848

Year： 2017

Issue： 12

Volume： 17

Page： 105-115

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SCOPUS Cited Count： 2

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Chinese Cited Count：

30 Days PV： 3

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