The　preparation　of　porous　polymethacrylate-based　open　tubular　capilary　columns　by　in-situ　copolymerization　of　octadecyl　methacrylate　(OMA),　and　ethylene　dimethacrylate　(EDMA)　in　N,N-dimethylformamide　(DMF)　solvent　are　proposed.　The　parameters　of　the　prep-　aration　procedure　of　the　stationary　phase　are　discussed　in　detail.　The　surface　of　the　cross-　section　of　an　open　tubular　(OT)　column　by　scanning　electron　microscope　(SEM)　showed　wrin-　kle　configuration,　which　is　expedient　to　enhance　proportion　of　stationary　phase　and　column　capacity.　Efficient　separation　of　six　alkyl　benzene　homologous　series　(namely,　toluene,　ethyl-　benzene,　propylbenzene,　butylbenzene,　amylbenzene　and　hexylbenzene)　were　performed　by　CEC　using　the　prepared　column,　and　their　elution　order　was　in　agreement　with　their　hydropho-　bicity.　So,　the　OT　columns　bonded　with　octadecyl　ligands　yielded　reversed-phase　retention　behavior　toward　nonpolar　solutes.　In　addition,　the　separation　of　four　antiepileptics　was　also　investigated　with　satisfactory　effectiveness.　The　column　efficiency　range　was　35　300-49　800　plates/m.　The　results　showed　that　the　C18-OT　column　of　organic　matrix　for　reversed-phase　chromatography　was　successfully　prepared　via　in-situ　polymerization　in　this　work.　Hereby,　this　new　column　thus　offers　a　promising　new　alternative　in　OT-CEC　and　will　be　useful　in　separation　science.