Rapid　detection　and　analytical　methods　of　rhodamine　B　were　established　in　food　by　ultra　high　performance　liquid　chromatograph.　Several　representative　foods　that　might　contain　rhodamine　B　were　used　to　test.　They　were　extracted　with　alkaline　dyes　pretreatment　reagent　kit,　purified　by　solid　phase　extraction　small　column　and　determined　with　ultra　high　performance　liquid　chromatography　fluorescence　detector.　The　results　show　that　the　method　is　suitable　for　the　analysis　of　rhodamine　B　in　tomato　sauce　and　the　optimal　sample　quality　is　5.0　g.　It　is　simple,　rapid　and　sensitive　method　to　detect　Rhodamine　B　in　food.　©,　2015,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.