A label-free fluorescent biosensor for ultratrace detection of terbium (sh{cyrillic}) based on structural conversion of G-quadruplex DNA mediated by ThT and terbium (sh{cyrillic}) - Details

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Abstract：

In　this　paper,　a　novel　label-free　fluorescent　biosensor　for　terbium　(sh{cyrillic})　(Tb3+)　was　proposed　based　on　structural　conversion　of　G-quadruplex　DNA　mediated　by　Thioflavin　T　(ThT)　and　Tb3+.　In　the　presence　of　K+,　ThT　could　bind　to　K+-stabilized　parallel　G-quadruplex,　giving　rise　to　high　fluorescence　intensity.　Upon　the　addition　of　Tb3+,　Tb3+　could　competitively　bind　to　parallel　G-quadruplex　leading　to　the　structural　change,　which　resulted　in　fluorescence　decrease.　The　change　of　fluorescence　intensity　(δF=F0-F)　showed　a　good　linear　response　toward　the　concentration　of　Tb3+　over　the　range　from　1.0pM　to　10.0μM　with　a　limit　of　detection　of　0.55pM.　This　proposed　biosensor　was　simple　and　cost-effective　in　design　and　in　operation　with　ultrahigh　sensitivity　and　selectivity.　Thus,　the　proposed　biosensor　could　be　a　promising　candidate　for　monitoring　ultratrace　Tb3+　in　environment.　©　2015　Elsevier　B.V.

Keyword：

Fluorescent biosensor; G-quadruplex; Terbium (sh{cyrillic}); Thioflavin T

Community：

[ 1 ] [Chen, Q.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 2 ] [Chen, Q.]Environmental Monitoring Center of Fujian Province, Fuzhou, Fujian, 350003, China
[ 3 ] [Zuo, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 4 ] [Chen, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 5 ] [Tong, P.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 6 ] [Mo, X.]Environmental Monitoring Center of Fujian Province, Fuzhou, Fujian, 350003, China
[ 7 ] [Zhang, L.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
[ 8 ] [Li, J.]Food Safety Key laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310035, China
[ 9 ] [Li, J.]Food Safety Key Laboratory of Liaoning Province, Bohai University, Jinzhou, 121013, China

Reprint 's Address：

[Zhang, L.]College of Chemistry, Fuzhou UniversityChina

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Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2015

Volume： 72

Page： 326-331

7 . 4 7 6

JCR@2015

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：265

JCR Journal Grade：1

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SCOPUS Cited Count： 18

ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 0

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