A　new　gold　particles　modified　heated　copper　disk　electrode　(Au-HCuDE)　with　direct　current　was　fabricated.　The　hemin/G-quadruplex　horseradish　peroxidase-mimicking　DNAzyme　(HRP-DNAzyme)　was　self-assembled　on　the　heated　electrode　and　resulted　in　a　new　biosensor　denoted　as　HRP-DNAzyme/Au-HCuDE.　By　controlling　the　temperature　of　the　surface　of　the　electrode,　the　dramatic　temperature　effect　on　the　electrocatalytic　reduction　of　H2O2　at　HRP-DNAzyme/Au-HCuDE　sensing　platform　was　demonstrated.　This　electrocatalytic　activity　of　HRP-DNAzyme　was　enhanced　with　electrode　temperature　elevated.　This　method　was　thus　preliminarily　used　to　develop　an　electrochemical　biosensor　for　highly　sensitive　detection　of　H2O2.　A　detection　limit　of　1.6×10-7M　could　be　obtained　(S/N=3)　with　an　electrode　temperature　of　50°C,　which　was　more　than　one　magnitude　lower　than　that　at　electrode　temperature　of　0°C.　This　heated　electrochemical　biosensor　shows　many　merits　such　as　easy　fabrication　and　simple　heating　equipment,　low　cost,　high　thermal　stability,　and　high　sensitivity　and　good　reproducibility.　©　2015　Elsevier　B.V.