Methods for genetic transformation of filamentous fungi - Details

author：

Li, D. (Li, D..) ^[1] | Tang, Y. (Tang, Y..) ^[2] | Lin, J. (Lin, J..) ^[3] | Cai, W. (Cai, W..) ^[4]

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Scopus

Abstract：

Filamentous　fungi　have　been　of　great　interest　because　of　their　excellent　ability　as　cell　factories　to　manufacture　useful　products　for　human　beings.　The　development　of　genetic　transformation　techniques　is　a　precondition　that　enables　scientists　to　target　and　modify　genes　efficiently　and　may　reveal　the　function　of　target　genes.　The　method　to　deliver　foreign　nucleic　acid　into　cells　is　the　sticking　point　for　fungal　genome　modification.　Up　to　date,　there　are　some　general　methods　of　genetic　transformation　for　fungi,　including　protoplast-mediated　transformation,　Agrobacterium-mediated　transformation,　electroporation,　biolistic　method　and　shock-wave-mediated　transformation.　This　article　reviews　basic　protocols　and　principles　of　these　transformation　methods,　as　well　as　their　advantages　and　disadvantages.　©　2017　The　Author(s).

Keyword：

Agrobacterium-mediated transformation; Biolistic method; Electroporation; Filamentous fungi; Protoplast-mediated transformation; Shock-wave-mediated transformation

Community：

[ 1 ] [Li, D.]Fuzhou University, Institute of Apply Genomics, No.2 Xueyuan Road, Fuzhou, 350108, China
[ 2 ] [Li, D.]Fuzhou University, College of Biological Science and Engineering, No.2 Xueyuan Road, Fuzhou, 350108, China
[ 3 ] [Tang, Y.]Triplex International Biosciences (China) Co. LTD, Xiamen, 361100, China
[ 4 ] [Lin, J.]Fuzhou University, Institute of Apply Genomics, No.2 Xueyuan Road, Fuzhou, 350108, China
[ 5 ] [Lin, J.]Fujian Medical University, School of Basic Medical Sciences, No.1 Xuefubei Road, Fuzhou, 350122, China
[ 6 ] [Lin, J.]Fuzhou University, College of Biological Science and Engineering, No.2 Xueyuan Road, Fuzhou, 350108, China
[ 7 ] [Cai, W.]Fuzhou University, Institute of Apply Genomics, No.2 Xueyuan Road, Fuzhou, 350108, China
[ 8 ] [Cai, W.]Fuzhou University, College of Biological Science and Engineering, No.2 Xueyuan Road, Fuzhou, 350108, China

Reprint 's Address：

[Lin, J.]Fuzhou University, Institute of Apply Genomics, No.2 Xueyuan Road, China

Email：

jun@fzu.edu.cn

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Source ：

Microbial Cell Factories

ISSN： 1475-2859

Year： 2017

Issue： 1

Volume： 16

3 . 8 3 1

JCR@2017

4 . 3 0 0

JCR@2023

ESI HC Threshold：231

JCR Journal Grade：1

CAS Journal Grade：2

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 147

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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