Laccases　can　oxidize　a　wide　range　of　aromatic　compounds　and　are　industrially　valuable.　Laccases　often　exist　in　gene　families　and　may　differ　from　each　other　in　expression　and　function.　Quantitative　real-time　polymerase　chain　reaction　(qPCR)　was　used　for　transcription　profiling　of　eight　laccase　genes　in　Cerrena　sp.　strain　HYB07　with　validated　reference　genes.　A　high　laccase　activity　of　280.0　U/mL　was　obtained　after　submerged　fermentation　for　5　days.　Laccase　production　and　laccase　gene　transcription　at　different　fermentation　stages　and　in　response　to　various　environmental　cues　were　revealed.　HYB07　laccase　activity　correlated　with　transcription　levels　of　its　predominantly　expressed　laccase　gene,　Lac7.　Cu2+　ions　were　indispensable　for　efficient　laccase　production　by　HYB07,　mainly　through　Lac7　transcription　induction,　and　no　aromatic　compounds　were　needed.　HYB07　laccase　synthesis　and　biomass　accumulation　were　highest　with　non-limiting　carbon　and　nitrogen.　Glycerol　and　inorganic　nitrogen　sources　adversely　impacted　Lac7　transcription,　laccase　yields,　and　fungal　growth.　The　present　study　would　further　our　understanding　of　transcription　regulation　of　laccase　genes,　which　may　in　turn　facilitate　laccase　production　as　well　as　elucidation　of　their　physiological　roles.　©　2016　Yang,　Wang,　Ng,　Lin　and　Ye.