[Objective]　Xylanase-producing　strains　were　screened　from　different　sources　of　samples.　The　strain　which　had　largest　xylanase　yield　was　identified　and　its　xylanase　properties　were　studied.　[Methods]　With　self-made　xylan　as　the　only　carbon　source,　the　xylnase-producing　strains　had　been　screened　according　to　the　transparent　zone　on　the　plate.　The　strain　was　identified　based　on　the　morphological　characteristics　observation　and　16S　rDNA　sequence　analysis.　[Results]　The　27　of　44　strains　which　showed　transparent　zones　had　xylanse　-producing　ability　and　B659　was　the　highest　among　them.　B659　was　identified　as　Bacillus　stratosphericus.　The　optimal　temperature　and　pH　of　B659　xylanse　was　55℃　and　6.5.78%　of　xylanse　activity　was　retained　after　2　h　incubation　at　37℃　and　pH　8-10　and　it　performed　great　alkali　resistance.　Besides,　10　mmol/L　K+　and　1　mmol/L　Fe2+　displayed　promotion　effect　to　xylnase　ability　while　Mn2+,　Cu2+　and　Co2+　manifested　obvious　inhibition.　[Conclusion]　Bacillus　stratosphericus　B659　with　high　ability　to　produce　alkali-resistance　xylanase　was　screened.　©　2016,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.