Herein,　functionalized　graphene　oxide　(GO)　was　prepared　by　the　covalent　functionalization　with　amino　acids　(lysine,　glycine,　glutamic　acid　and　tyrosine)　in　this　study.　Zeta　potential　results　demonstrated　that　covalent　functionalization　of　GO　with　amino　acids　was　favourable　for　their　homogeneous　dispersion　in　water　and　organic　solvents.　Based　on　the　higher　absolute　value　of　zeta　potential　and　the　better　dipersion　stability　of　GO-lysine,　the　PVDF/GO-lysine　hybrid　membranes　were　then　prepared　via　the　phase　inversion　induced　by　immersion　precipitation　technique.　SEM　images　showed　a　better　pore　diameter　and　porosity　distribution　on　the　PVDF/GO-lysine　membrane　surface.　The　zeta　potential　absolute　value　of　the　PVDF/GO-lysine　membrane　surface　was　higher　than　that　of　the　virgin　PVDF　membrane.　Furthermore,　the　PVDF/GO-lysine　membranes　surface　exhibited　good　hydrophilicity.　The　water　flux　of　PVDF/GO-lysine　membranes　can　reach　to　two　times　of　that　of　the　virgin　PVDF　membrane.　And　the　BSA　adsorbed　amount　on　PVDF/GO-lysine　surface　was　decreased　to　0.82　mg/cm2　for　PVDF/GO-lysine-8%　membrane.　Filtration　experiment　results　indicated　that　the　fouling　resistance　was　significantly　improved　for　the　PVDF/GO-lysine　membranes.　As　a　result,　lysine　functionalized　GO　will　provide　a　promising　method　to　fabricate　graphene　oxide　based　hybrid　membranes　with　effective　antifouling　property　and　hydrophilicity.　©　2016,　Chinese　Chemical　Society,　Institute　of　Chemistry,　Chinese　Academy　of　Sciences　and　Springer-Verlag　Berlin　Heidelberg.