Organogel　can　solidify　and　trap　liquid　oil　and　showed　a　solid-like　rheological　property,　and　the　application　of　organogels　to　structure　liquid　oils　had　become　an　active　area　of　research　in　the　past　years.　In　this　study,　we　examined　the　effect　of　bonding　mixture　of　β-sitosterol　and　γ-oryzanolas　as　organogel　on　animal　nutrition　physiological　function　by　the　rat　feeding　test,　including　the　high　risk　factor　of　cardiovascular　disease.　With　feed　of　margarine　structured　by　bonding　mixture　of　β-sitosterol　and　γ-oryzanolas,　there　was　no　adverse　effects　on　rats.　The　results　showed　that,　rat　body　weight　of　experimental　groups　was　lower　than　the　control　group,　and　the　high　dose　group　was　the　lowest.　Meanwhile,　the　data　implied　that　the　increasing　dose　of　sterol　organogel　can　promote　accumulation　of　adipose　tissue　in　the　part　of　testosterone　and　kidney　of　rats.　In　addition,　the　rats　serum　total　cholesterol,　triglyceride　and　low　density　lipoprotein　levels　were　decreased　with　the　increasing　amount　of　organogel,　which　indicated　that　sterol　organogel　had　better　effect　on　the　risk　of　antiatherosclerosis.　©　2016,　Dept.　of　JCCOA.　All　right　reserved.