A　liquid　chromatography　coupled　with　electrospray　ionization　tandem　mass　spectrometry　(LC-ESI-MS/MS)　method　based　on　dispersive　solid-phase　extraction　was　developed　for　the　simultaneous　determination　of　three　fungicides　residues　in　mushrooms,　including　carbendazim,　diethofencarb　and　prochloraz.　Target　pesticides　were　extracted　from　simples　with　acetonitrile　containing　1%　acetic　acid　and　purified　by　QuEChERS　method.　The　three　compounds　were　separated　on　a　Waters　Atlantis®　T3　column　with　the　mobile　phases　of　acetonitrile　and　5　mmol/L　ammonium　acetate　containing　0.1%　formic　acid.　Target　compounds　were　analyzed　by　MS/MS　with　positive　electrospray　ionization　in　multiple　reaction　monitoring　(MRM)　mode,　and　quantified　by　external　standard　method.　The　results　demonstrated　that　the　method　showed　good　linearity　in　the　range　of　1.0~25　μg/L　for　3　fungicides　with　correlation　coefficients　no　less　than　0.9947.　The　limits　of　detection　(S/N=3)　were　0.08-0.33　μg/kg　and　the　limits　of　detection　(S/N=10)　were　0.27~1.2　μg/kg.　The　mean　recoveries　of　3　fungicides　from　two　kinds　of　samples　spiked　with　three　concentration　levels　of　8,　40,　100　μg/kg　ranged　from　79.3%　to　111.7%　with　relative　standard　deviations　(RSDs)　of　1.5%-9.3%.　The　method　is　efficient,　sensitive,　accurate　and　suitable　for　simultaneous　determination　of　these　pesticides　in　mushrooms.　©　2016,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.