In　this　paper,　a　novel　highly　cross-linked　porous　monolithic　stationary　phase　having　a　long　alkyl　chain　ligand　(C16)　was　introduced　and　evaluated　in　CEC.　The　monolithic　stationary　phase　was　prepared　by　in　situ　copolymerization　of　1-hexadecene,　trimethylolpropane　trimethacrylate,　and　2-acrylamido-2-methyl-1-　propanesulfonic　acid　(AMPS)　in　the　presence　of　ternary　porogenic　solvent　(cyclohexanol/1,4-butanediol/water).　In　preparing　monoliths,　the　ternary　cross-linker　trimethylolpropane　trimethacrylate　was　usually　applied　to　preparing　molecularly　imprinted　polymers　or　molecularly　imprinted　solid-phase　extraction,　instead　of　binary　cross-linker　ethylene　dimethacrylate.　1-Hexadecene　was　introduced　to　provide　the　non-polar　sites　(C16)　for　chromatographic　retention,　while　AMPS　was　used　to　generate　the　EOF　for　transporting　the　mobile　phase　through　the　monolithic　capillary.　Monolithic　columns　were　prepared　by　optimizing　proportion　of　porogenic　solvent　and　AMPS　content　in　the　polymerization　solution　as　well　as　the　cross-linkers.　The　monolithic　stationary　phases　could　generate　a　strong　and　stable　EOF　in　various　pH　values　and　exhibit　an　RP-chromatographic　behavior　for　neutral　compounds.　For　charged　compounds,　the　separation　was　mainly　based　on　the　association　of　hydrophobic,　electrostatic　and　electrophoretic　interaction.　©　2009　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA.