Novel　fluorescence　and　SERS　encoded　microspheres　based　on　poly(glycidyl　methacrylate)　(PGMA)　microspheres　were　developed　by　a　controllable　approach.　The　microspheres　were　encoded　by　four　fluorescence　dyes　and　three　surface　enhanced　Raman　spectra　(SERS)　probes.　Multiple　dyes　could　be　simultaneously　incorporated　into　the　PGMA　microspheres　for　encoding　and　the　intensity　of　the　fluorescence　can　be　tuned　by　adjusting　the　feeding　ratio.　The　PGMA　microspheres　were　coated　by　silver　nanoparticles　and　encoded　by　single　and　multiple　SERS　reporters.　The　fluorescence　and　SERS　joint　encoded　PGMA　microspheres　were　fabricated.　All　the　fluorescence　encoding　signals　can　be　excited　at　488　nm.　The　fluorescent　and　SERS　signals　of　dual-mode　microspheres　could　be　obtained　from　two　optical　channels　and　non-overlapping　of　emission　spectra　was　observed　in　these　encoded　microspheres,　which　greatly　increased　the　encoding　capacity.　The　as　prepared　fluorescence　and　SERS　encoded　microspheres　possess　stable　and　distinct　spectral　encoding　signals,　large　encoding　capacity.　©　BME-PT.