Opposite salt-dependent stability of i-motif and duplex reflected in a single DNA hairpin nanomachine - Details

author：

Zhang, F. (Zhang, F..) ^[1] | Liu, B. (Liu, B..) ^[2] | Lopez, A. (Lopez, A..) ^[3] | Wang, S. (Wang, S..) ^[4] | Liu, J. (Liu, J..) ^[5]

Indexed by：

Scopus

Abstract：

We　herein　report　a　DNA　hairpin　structure　containing　a　polycytosine　loop　region,　and　this　hairpin　can　operate　like　a　nanomachine　allowing　independently　controlled　stability　of　the　i-motif　loop　and　duplex　stem　region.　This　was　made　possible　by　the　opposite　salt-dependent　stability　of　DNA　duplex　and　hairpin,　thus　providing　a　new　method　for　designing　molecular　devices　or　switches　design.　A　singly-labeled　fluorescent　method　was　used　to　measure　the　stability　of　an　i-motif　DNA　in　the　presence　of　various　metal　ions.　Salt　in　general　destabilizes　the　i-motif　but　stabilizes　duplex　DNA,　allowing　us　to　engineer　an　i-motif　containing　hairpin　for　modulating　the　stability　of　each　secondary　structure　independently.　©　2020　IOP　Publishing　Ltd.

Keyword：

Community：

[ 1 ] [Zhang, F.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China
[ 2 ] [Zhang, F.]Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
[ 3 ] [Liu, B.]Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
[ 4 ] [Lopez, A.]Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
[ 5 ] [Wang, S.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China
[ 6 ] [Liu, J.]Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada

Reprint 's Address：

[Zhang, F.]College of Biological Science and Engineering, Fuzhou UniversityChina

Email：

Show more details

Related Keywords：

Source ：

Nanotechnology

ISSN： 0957-4484

Year： 2020

Issue： 19

Volume： 31

3 . 8 7 4

JCR@2020

2 . 9 0 0

JCR@2023

ESI HC Threshold：196

JCR Journal Grade：2

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 7

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

Affiliated Colleges：

Get Fulltext

DOI Library Discovery Baidu Scholar Search SCOPUS

Type
Departments

All Years Choose Year From to