Construction of a Target-Initiated, Enzyme-Free DNA Cascade Circuit for Amplified Detection of Mercury - Details

author：

Xing, C. (Xing, C..) ^[1] | Chen, Z. (Chen, Z..) ^[2] | Zhang, C. (Zhang, C..) ^[3] | Wang, J. (Wang, J..) ^[4] | Lu, C. (Lu, C..) ^[5]

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Scopus

Abstract：

Here,　we　designed　a　target-initiated,　enzyme-free　DNA　cascade　circuit　for　highly　selective　and　sensitive　detection　of　Hg2+　with　dual　amplification　based　on　concatenated　fuel　stimulant　powered　(FSP)　and　catalytic　hairpin　assembly　(CHA)　circuits.　In　a　typical　FSP-CHA　procedure,　in　the　presence　of　Hg2+,　the　invading　DNA　activated　the　upstream　FSP　circuit　to　release　the　hidden　strands　containing　multiple　trigger　units,　which　subsequently　motivated　the　downstream　CHA　circuit　to　drive　the　cross-opening　of　two　hairpin　substrates,　generating　tremendously　amplified　fluorescence　signals.　A　good　linearity　between　the　fluorescence　intensity　and　Hg2+　concentrations　was　observed　in　the　range　from　0.2　nM　to　20　nM.　The　proposed　system　showed　great　applicability　for　sensing　Hg2+　in　environmentally　relevant　water　samples.　Copyright　©　2020　American　Chemical　Society.

Keyword：

catalytic hairpin assembly; DNA circuit; enzyme-free; fuel stimulant powered; mercury

Community：

[ 1 ] [Xing, C.]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, Minjiang University, Fuzhou, 350108, China
[ 2 ] [Chen, Z.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 3 ] [Zhang, C.]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, Minjiang University, Fuzhou, 350108, China
[ 4 ] [Wang, J.]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, Minjiang University, Fuzhou, 350108, China
[ 5 ] [Lu, C.]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China

Reprint 's Address：

[Xing, C.]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou UniversityChina

Email：

chaoxing@mju.edu.cn

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Source ：

ACS Applied Bio Materials

ISSN： 2576-6422

Year： 2020

Issue： 4

Volume： 3

Page： 1853-1857

4 . 7 0 0

JCR@2023

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 1

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