We　developed　dual　biologically　responsive　nanogapped　gold　nanoparticle　vesicles　loaded　with　immune　inhibitor　and　carrying　an　anticancer　polymeric　prodrug　for　synergistic　concurrent　chemo-immunotherapy　against　primary　and　metastatic　tumors,　along　with　guided　cargo　release　by　photoacoustic　(PA)　imaging　in　the　second　near-infrared　(NIR-II)　window.　The　responsive　vesicle　was　prepared　by　self-assembly　of　nanogapped　gold　nanoparticles　(AuNNPs)　grafted　with　poly(ethylene　glycol)　(PEG)　and　dual　pH/GSH-responsive　polyprodug　poly(SN38-co-4-vinylpyridine)　(termed　AuNNP@PEG/PSN38VP),　showing　intense　PA　signal　in　the　NIR-II　window.　The　effect　of　the　rigidity　of　hydrophobic　polymer　PSN38VP　on　the　assembled　structures　and　the　formation　mechanism　of　AuNNP@SN38　Ve　were　elucidated　by　computational　simulations.　The　immune　inhibitor　BLZ-945　was　encapsulated　into　the　vesicles,　resulting　in　pH-responsive　release　of　BLZ-945　for　targeted　immunotherapy,　followed　by　the　dissociation　of　the　vesicles　into　single　AuNNP@PEG/PSN38VP.　The　hydrophilic　AuNNP@PEG/PSN38VP　nanoparticles　could　penetrate　deep　into　the　tumor　tissues　and　release　the　anticancer　drug　SN38　under　the　reductive　environment.　A　PA　signal　in　the　NIR-II　window　in　the　deep　tumor　region　was　obtained.　The　BLZ-945-loaded　vesicle　enabled　enhanced　PA　imaging-guided　concurrent　chemo-immunotherapy　efficacy,　inhibiting　the　growth　of　both　primary　tumors　and　metastatic　tumors.