The　environmental　behaviors　of　steroid　estrogens　(SEs)　associated　with　land　irrigation　and　application　are　of　critical　concern　worldwide.　Understanding　the　spatio-temporal　distribution　and　transformation　process　of　these　estrogenic　compounds　in　soil　is　greatly　significant.　In　this　study,　laboratory　soil　column　experiments　were　conducted　to　investigate　and　explore　the　migration　and　abiotic　transformation　of　17α-estradiol　(17α-E2)　and　17β-estradiol　(17β-E2)　over　spatial　and　time　scales.　Results　indicated　that　the　migration　tendency　of　17α-E2　and　17β-E2　was　similar.　Discrepancies　in　transport　for　different　SEs　groups　might　be　due　to　the　competitive　sorption　and　isomeric　transformation　in　the　binary-solute　system.　17α-E2　and　17β-E2　can　also　undergo　the　abiotic　transformation　during　soil　column　transport.　The　soil　with　naturally　abundant　mineral　substances　(e.g.,　iron　and　manganese　oxides)　indicated　that　E2　isomers　tended　to　mineral-promoted　racemization,　oxidation,　reduction,　and　radical　coupling　reactions.　Some　possible　transformation　products　(e.g.,　SE239,　E2378,　and　SE　dimer476)　were　identified　and　proposed　in　soil　samples.　Compared　to　the　single　compound　tests,　the　estimated　17β-estradiol　equivalency　(EEQ)　values　of　E2　mixture　were　higher　during　SEs　migration　process.　©　2020　Elsevier　B.V.