In　this　work,　a　novel　method　based　on　gold　nanoparticle　preconcentration　coupled　with　CE　for　electrochemiluminescence　detection　of　ciprofloxacin,　enrofloxacin,　ofloxacin,　and　norfloxacin　in　European　eels　was　developed.　The　addition　of　gold　nanoparticles　induced　the　rapid　enrichment　of　fluoroquinolones,　which　was　simpler　than　the　conventional　enrichment　approaches　such　as　solid　phase　extraction　and　solid-phase　microextraction.　More　than　100　times　enrichment　was　observed　after　gold　nanoparticle　aggregation-based　preconcentration.　The　CE-electrochemiluminescence　parameters　that　affected　the　separation　and　detection　were　optimized.　Under　the　optimized　conditions,　the　linear　ranges　for　the　four　fluoroquinolones　were　0.090-8.0　μmol　L-1　with　the　detection　limits　between　0.020　and　0.050　μmol　L-1.　The　proposed　approach　showed　the　advantages　of　high　sensitivity,　high　selectivity,　a　wide　linear　range,　and　a　low　detection　limit.　It　was　used　to　analyze　fluoroquinolones　in　European　eel,　and　the　results　showed　that　the　developed　method　can　satisfy　the　detection　requirements　for　fluoroquinolone　determination　in　aquatic　products　set　by　China　and　the　European　Union.　©　2020　The　Royal　Society　of　Chemistry.