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[期刊论文]

Rolling circle amplification promoted magneto-controlled photoelectrochemical biosensor for organophosphorus pesticides based on dissolution of core-shell MnO2 nanoflower@CdS mediated by butyrylcholinesterase

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author:

Tang, J. (Tang, J..) [1] | Li, J. (Li, J..) [2] | Xiong, P. (Xiong, P..) [3] | Unfold

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Scopus

Abstract:

A photoelectrochemical (PEC) aptasensing platform is devised for sensitive detection of an organophosphorus pesticide based on dissolution of core-shell MnO2 nanoflower@CdS (MnO2 NF@CdS) by thiocholine (TCh). TCH is produced from the butyrylcholinesterase-acetylthiocholine system, accompanied by target-triggered rolling circle amplification (RCA). The core-shell MnO2 NF@CdS with excellent PEC performance was synthesized and employed as a photo-sensing platform. The target was detected on a functionalized magnetic probe with the corresponding aptamer. Upon malathion introduction, the aptamer was detached from the magnetic beads, while capture DNA (cDNA, with primer fragment) remained on the beads. The primer fragment in cDNA can trigger the RCA reaction to form a long single-stranded DNA (ssDNA). Furthermore, a large number of butyrylcholinesterase (BChE) were assembled on the long ssDNA strands through the hybridization with the S2-Au-BChE probe. Thereafter, TCh generated from hydrolysis of ATCh by BChE can reduce MnO2 NF (core) to Mn2+ and release the CdS nanoparticles (shell) from the platform electrode, significantly enhancing the PEC signal. Under optimal conditions, the proposed aptasensor exhibited high sensitivity for malathion with a low detection limit of 0.68 pg mL−1. Meanwhile, it also presents outstanding specificity, reproducibility, and stability. Importantly, the sensing platform provides a new concept for detection of pesticide. [Figure not available: see fulltext.]. © 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

Keyword:

Core-shell MnO2 NF@CdS nanostructure; Malathion; Photoelectrochemical biosensor; Rolling circle amplification

Community:

  • [ 1 ] [Tang, J.]Ministry of Education Key Laboratory of Functional Small Organic Molecule, Department of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang, 330022, China
  • [ 2 ] [Li, J.]Ministry of Education Key Laboratory of Functional Small Organic Molecule, Department of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang, 330022, China
  • [ 3 ] [Xiong, P.]Ministry of Education Key Laboratory of Functional Small Organic Molecule, Department of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang, 330022, China
  • [ 4 ] [Sun, Y.]Ministry of Education Key Laboratory of Functional Small Organic Molecule, Department of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang, 330022, China
  • [ 5 ] [Zeng, Z.]Ministry of Education Key Laboratory of Functional Small Organic Molecule, Department of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang, 330022, China
  • [ 6 ] [Tian, X.]Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China
  • [ 7 ] [Tang, D.]Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education of China and Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, China

Reprint 's Address:

  • [Tang, J.]Ministry of Education Key Laboratory of Functional Small Organic Molecule, Department of Chemistry and chemical engineering, Jiangxi Normal UniversityChina

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Source :

Microchimica Acta

ISSN: 0026-3672

Year: 2020

Issue: 8

Volume: 187

5 . 8 3 3

JCR@2020

5 . 4 0 0

JCR@2023

ESI HC Threshold:160

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 26

30 Days PV: 1

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