Lutein　is　a　metabolite　produced　by　plants　or　microalgae,　and　offers　various　health　benefits.　Chlorella　sorokiniana　FZU60　is　considered　a　hyper-lutein　producing　microalga.　In　this　study,　the　effects　of　temperature　and　light　intensity　on　cell　growth　and　lutein　accumulation　by　FZU60　under　mixotrophic　conditions　were　investigated.　Maximal　lutein　productivity　and　production　were　obtained　at　33　°C,　and　high　light　intensity　enhanced　cell　concentration　but　decreased　lutein　content　when　nitrate　was　replete.　Further,　two　fed-batch　strategies　with　gradient　(fed-batch　strategy　I)　or　constant　(fed-batch　strategy　II)　NaAc　feeding　concentrations　were　compared.　Results　showed　that　the　second　strategy　was　more　desirable　for　lutein　production.　Moreover,　two　types　of　mixotrophy/photoautotrophy　two-stage　strategies　with　a　light　intensity　of　750　μmol/m2/s　(two-stage　strategy　I)　or　150　μmol/m2/s　(two-stage　strategy　II)　in　stage　2　were　explored.　Under　the　two-stage　strategy　I,　high　lutein　content　(11.22　mg/g),　productivity　(8.25　mg/L/d),　and　production　(65.96　mg/L)　were　achieved.　The　lutein　content,　productivity,　and　production　obtained　in　this　study　are　higher　than　those　reported　in　most　related　studies,　indicating　that　the　proposed　strategy　can　be　used　in　commercial　production.　©　2020　Elsevier　B.V.